Human Anti-MET Recombinant Antibody (PABL-271)
CAT#: PABL-271
Recombinant Human Antibody is capable of binding to MET, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-MET mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-MET mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody potently inhibits HGF binding and receptor phosphorylation and signaling and has antibody-like pharmacokinetics and antitumor activity.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Human Anti-MET Recombinant Antibody (PABL-271) in SDS-PAGE
SDS-PAGE analysis of PABL-271 in non-reduced (Lane 1, 2 μg) and reduced (Lane 2, 2 μg) conditions.
SEC-HPLC
Figure 2 Human Anti-MET Recombinant Antibody (PABL-271) in HPLC
The purity of PABL-271 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-MET Recombinant Antibody (PABL-271) in WB
Western blot analysis of PABL-271 was performed by loading recombinant human MET.
PABL-271 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-human IgG
Lane 1: Reduced Antigen (0.3 μg)
Lane 2: Reduced Antigen (0.6 μg)
ELISA
Figure 4 Human Anti-MET Recombinant Antibody (PABL-271) in ELISA
ELISA analysis of PABL-271 was performed by coating with recombinant human MET.
The secondary antibody: HRP-anti-human IgG
DB
Figure 5 Human Anti-MET Recombinant Antibody (PABL-271) in DB
Dot Blot analysis of PABL-271 was performed by coating with recombinant human MET.
The secondary antibody: HRP-anti-human IgG
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55607/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18577/if_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A-431, CAPAN-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105976-MET
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human MET
- Species Reactivity
- Human
- Applications
- WB, Block, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MET; MET proto-oncogene; receptor tyrosine kinase; HGFR; AUTS9; RCCP2; c-Met; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
- Gene ID
- 4233
- UniProt ID
- A0A024R759
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-271, RRID: AB_3111642)
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| TAB-534LC | Mouse Anti-MET Recombinant Antibody (TAB-534LC) | FC, FuncS, Inhib | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-536LC | Human Anti-MET Recombinant Antibody (TAB-536LC) | Inhib | Human IgG2 |
| TAB-537LC | Human Anti-MET Recombinant Antibody (TAB-537LC) | Inhib | Human IgG2 |
| TAB-538LC | Human Anti-MET Recombinant Antibody (TAB-538LC) | Inhib | Human IgG2 |
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| TAB-002MZ | Anti-Human MET Recombinant Antibody (TAB-002MZ) | IHC, PET imaging | Chimeric antibody (mouse/human) |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOR-4645 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH158DS) | WB, IF, ICC, FC, ELISA | IgG |
| MOR-4694 | Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH208DS) | WB, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YC | Mouse Anti-MET Recombinant Antibody (HPAB-0071-YC) | ELISA, Inhib | Mouse IgG |
| HPAB-0072-YC | Human Anti-MET Recombinant Antibody (HPAB-0072-YC) | ELISA | Humanized IgG1, κ |
| HPAB-0073-YC | Human Anti-MET Recombinant Antibody (HPAB-0073-YC) | ELISA | Human IgG1, κ |
| HPAB-0077-YC | Mouse Anti-MET Recombinant Antibody (clone LMH 89) | ELISA, FC, Stim, FuncS | Mouse IgG |
| HPAB-1298LY | Human Anti-MET Recombinant Antibody (HPAB-1298LY) | ELISA, IP | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0760-CN-F(E) | Mouse Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0760-CN-F(E)) | WB, ELISA, IHC, IP | Mouse Fab |
| HPAB-0761-CN-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0761-CN-F(E)) | WB, ELISA, IHC, IP | Human Fab |
| HPAB-0762-CN-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0762-CN-F(E)) | WB, ELISA, IHC, IP | Human Fab |
| HPAB-M0277-YC-F(E) | Human Anti-MET Recombinant Antibody (clone ch3E1D7); Fab Fragment | ELISA, Neut | Human Fab |
| HPAB-0117-WJ-F(E) | Mouse Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0117-WJ-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1298LY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-1298LY-S(P)) | ELISA, IP | Human scFv |
| HPAB-1299LY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-1299LY-S(P)) | ELISA, IP | Human scFv |
| HPAB-1300LY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-1300LY-S(P)) | ELISA, IP | Human scFv |
| HPAB-1301LY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-1301LY-S(P)) | ELISA, IP | Human scFv |
| HPAB-1302LY-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-1302LY-S(P)) | ELISA, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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